Description
Product Information
| Purity: | ≥98% for each compound |
| Storage: | 4oC for 3 month. -20oC for 6 month. |
| Cocktail Formulation: | 5 mM Thiazovivin, 2 mM SB-431542, 0.4 mM PD0325901 and 1 mM CHIR99021 in DMSO. |
Biological Activity:
Extensive studies have shown that individual Rock inhibitor (Thiazovivin), TGFβ inhibitor (SB431542), MEK inhibitor (PD0325901), GSK3β inhibitor (CHIR99021), , and their combinations (TSPC) can significantly enhance reprogramming efficiency of human somatic cells to induced pluripotent stem cells (iPSCs). For customers’ convenience, XcessBio has formulated the ready-to-use iPSC-TSPC cocktail containing all four small molecules at optimal concentrations and functionally tested it in iPSC reprogramming.
How to Use:
- In vitro: Dilute the received ready-to-use iPSC-TSPC cocktail at 1:1000 into your iPSC reprogramming medium to make the iPSC-TSPC media for iPSC induction.
Reference:
- Valamehr B, et al. A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. (2012) SCIENTIFIC REPORTS. 2 : 213
- Li W, et al. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. (2009) Stem Cells. 27(12):2992-3000.
- Lin T, et al. A chemical platform for improve induction of human iPS cells. (2009) Nature Method 6, 805-808.
- Kawamata M, et al. Generation of genetically modified rats from embryonic stem cells. (2010) PNAS 107, 14223-14228.
- Zhu S, et al. Reprogramming of human primary somatic cells by OCT4 and chemical compounds.(2010) Cell Stem Cell 7, 651-655.
- Yu J, et al. Efficient feeder-free episomal reprogramming with small molecules. (2011) PLoS ONE 6(3): e17557.
Products are for research use only. Not for human use.
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