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iPSC-TSPC

iPSC-TSPC

$109.00
Combination of Thiazovivin (ROCK inhibitor), SB431542 (TGFβ inhibitor), PD0325901 (MEK inhibitor), and CHIR99021 (GSK3 inhibitor) can enhance and accelerate reprogramming of human somatic cells (e.g., fibroblasts) into iPSCs.
Catalog No. Unit Price Qty
S10005 1000X; 0.5 ml $109.00

Details

Product Information
Purity: ≥98% for each compound
Storage: 4oC for 3 month. -20oC for 6 month.
Cocktail Formulation: 5 mM Thiazovivin, 2 mM SB-431542, 0.4 mM PD0325901 and 1 mM CHIR99021 in DMSO.
Effective Concentration in Cell Culture: 5 µM Thiazovivin, 2 µM SB-431542, 0.4 µM PD0325901 and 1 µM CHIR99021 in cell culture.

Biological Activity:

Extensive studies have shown that individual Rock inhibitor (Thiazovivin), TGFβ inhibitor (SB431542), MEK inhibitor (PD0325901), GSK3β inhibitor (CHIR99021), , and their combinations (TSPC) can significantly enhance reprogramming efficiency of human somatic cells to induced pluripotent stem cells (iPSCs). For customers’ convenience, XcessBio has formulated the ready-to-use iPSC-TSPC cocktail containing all four small molecules at optimal concentrations and functionally tested it in iPSC reprogramming.

How to Use:

  • In vitro: Dilute the received ready-to-use iPSC-TSPC cocktail at 1:1000 into your iPSC reprogramming medium to make the iPSC-TSPC media for iPSC induction.

Reference:

  1. 1.  Valamehr B, et al.  A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. (2012) SCIENTIFIC REPORTS. 2 : 213
  2. 2.  Li W, et al. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. (2009) Stem Cells. 27(12):2992-3000.
  3. 3.  Lin T, et al. A chemical platform for improve induction of human iPS cells. (2009) Nature Method 6, 805-808.
  4. 4.  Kawamata M, et al. Generation of genetically modified rats from embryonic stem cells. (2010) PNAS 107, 14223-14228.
  5. 5.  Zhu S, et al. Reprogramming of human primary somatic cells by OCT4 and chemical compounds.(2010)  Cell Stem Cell 7, 651-655.
  6. 6.  Yu J, et al. Efficient feeder-free episomal reprogramming with small molecules. (2011) PLoS ONE 6(3): e17557.




Products are for research use only. Not for human use.

Description

Details

Product Information
Purity: ≥98% for each compound
Storage: 4oC for 3 month. -20oC for 6 month.
Cocktail Formulation: 5 mM Thiazovivin, 2 mM SB-431542, 0.4 mM PD0325901 and 1 mM CHIR99021 in DMSO.
Effective Concentration in Cell Culture: 5 µM Thiazovivin, 2 µM SB-431542, 0.4 µM PD0325901 and 1 µM CHIR99021 in cell culture.

Biological Activity:

Extensive studies have shown that individual Rock inhibitor (Thiazovivin), TGFβ inhibitor (SB431542), MEK inhibitor (PD0325901), GSK3β inhibitor (CHIR99021), , and their combinations (TSPC) can significantly enhance reprogramming efficiency of human somatic cells to induced pluripotent stem cells (iPSCs). For customers’ convenience, XcessBio has formulated the ready-to-use iPSC-TSPC cocktail containing all four small molecules at optimal concentrations and functionally tested it in iPSC reprogramming.

How to Use:

  • In vitro: Dilute the received ready-to-use iPSC-TSPC cocktail at 1:1000 into your iPSC reprogramming medium to make the iPSC-TSPC media for iPSC induction.

Reference:

  1. 1.  Valamehr B, et al.  A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. (2012) SCIENTIFIC REPORTS. 2 : 213
  2. 2.  Li W, et al. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. (2009) Stem Cells. 27(12):2992-3000.
  3. 3.  Lin T, et al. A chemical platform for improve induction of human iPS cells. (2009) Nature Method 6, 805-808.
  4. 4.  Kawamata M, et al. Generation of genetically modified rats from embryonic stem cells. (2010) PNAS 107, 14223-14228.
  5. 5.  Zhu S, et al. Reprogramming of human primary somatic cells by OCT4 and chemical compounds.(2010)  Cell Stem Cell 7, 651-655.
  6. 6.  Yu J, et al. Efficient feeder-free episomal reprogramming with small molecules. (2011) PLoS ONE 6(3): e17557.




Products are for research use only. Not for human use.

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