FPH1 --- Hepatocytes Functional Proliferation Inducer

FPH1 is a potent and selective small molecule that induced Functional Proliferation of primary human Hepatocytes in vitro, identified by a high-throughput, cell-based screening using primary human hepatocytes. It induced a significant increase in hepatocyte number and elevated the number of hepatocytes undergoing mitosis in a dose-dependent manner. Cells treated with FPH1 also maintained their liver-specific functions. Immunofluorescence staining for Ki67 and albumin, along with FACS-based cell counting, revealed that hepatocytes from a wide range of genetically diverse individuals could be expanded and normal hepatocytes functions could be maintained after expansion. FPH1 can also promote the differentiation of iPS cells toward a hepatic lineage and promote the maturation of induced hepatic cells toward a more adult-like liver phenotype.

Product information

CAS Number: 708219-39-0

Molecular Weight: 388.82

Formula: C16H15ClF2N2O3S

Synonym:

BRD-6125

Chemical Name: 2-(N-(5-chloro-2-methylphenyl)methylsulfonamido)-N-(2,6-difluorophenyl)acetamide

Smiles: CC1=CC=C(Cl)C=C1N(CC(=O)NC1C(F)=CC=CC=1F)S(C)(=O)=O

InChiKey: WAOBCCBUTHNTFO-UHFFFAOYSA-N

InChi: InChI=1S/C16H15ClF2N2O3S/c1-10-6-7-11(17)8-14(10)21(25(2,23)24)9-15(22)20-16-12(18)4-3-5-13(16)19/h3-8H,9H2,1-2H3,(H,20,22)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

FPH1 was suggested to be supplemented into the culture medium on day 1 and 5 at a concentration of 15-20 μM to induce functional proliferation of primary human hepatocytes in vitro.

References:

1. Shan J, et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. (2013) Nat Chem Biol. 9(8):514-20.

Products are for research use only. Not for human use.

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