LY411575 --- γ-secretase Inhibitor

LY411575 is a highly potent, selective, and orally bioavailable inhibitor of gamma secretase with an IC50 of ~0.1 nM for APP23 and APP51/16 in primary neurons. It has been shown to promote goblet cell differentiation in mouse intestine. LY411575 has also been observed to promote neural differentiation of mouse embryonic stem (ES) cells. In recent studies, LY411575 increased hair cell number in organ of Corti explants. Systemic LY411575 administration increased hair cell number and promoted hearing recovery in a noise-damaged cochlea, but local LY411575 administration promoted hearing recovery through transdifferentiation of supporting cells into hair cells after noise-induced hearing loss in the mature cochlea.

Product information

CAS Number: 209984-57-6

Molecular Weight: 479.48

Formula: C26H23F2N3O4

Synonym:

LY-411575

LY 411575

LSN-411575

LSN 411575

LSN411575

Chemical Name: (S)-2-((S)-2-(3, 5-difluorophenyl)-2-hydroxyacetamido)-N-((S)-5-methyl-6-oxo-6, 7-dihydro-5H-dibenzo[b, d]azepin-7-yl)propanamide

Smiles: C[C@H](NC(=O)[C@@H](O)C1=CC(F)=CC(F)=C1)C(=O)N[C@@H]1C(=O)N(C)C2=CC=CC=C2C2=CC=CC=C21

InChiKey: ULSSJYNJIZWPSB-CVRXJBIPSA-N

InChi: InChI=1S/C26H23F2N3O4/c1-14(29-25(34)23(32)15-11-16(27)13-17(28)12-15)24(33)30-22-20-9-4-3-7-18(20)19-8-5-6-10-21(19)31(2)26(22)35/h3-14,22-23,32H,1-2H3,(H,29,34)(H,30,33)/t14-,22-,23-/m0/s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

LY411575 was usually used at 10 µM final concentration in vitro and in cellular assays.

In Vivo:

LY411575 was orally dosed to mice at 10-50mg/kg once per day or intraperitoneally dosed to mice at 5 mg/kg once per day.

References:

1. Wong GT, et al. Chronic treatment with the gamma-secretase inhibitor LY-411,575 inhibits beta-amyloid peptide production and alters lymphopoiesis and intestinal cell differentiation. (2004) J Biol Chem. 279(13):12876-82.
2. Dev KK, et al. Signal peptide peptidase dependent cleavage of type II transmembrane substrates releases intracellular and extracellular signals. (2006) Eur J Pharmacol. 540(1-3):10-7.
3. Fuwa H,et al. Divergent synthesis of multifunctional molecular probes to elucidate the enzyme specificity of dipeptidic gamma-secretase inhibitors. (2007) ACS Chem Biol. 2(6):408-18.
4. Spilman P, et al. A gamma-secretase inhibitor and quinacrine reduce prions and prevent dendritic degeneration in murine brains. (2008) Proc Natl Acad Sci U S A. 105(30):10595-600.
5. Abranches E, et al. Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo. (2009) PLoS One. 4(7):e6286.
6. Mizutari K, et al. Notch inhibition induces cochlear hair cell regeneration and recovery of hearing after acoustic trauma. (2013) Neuron. 77(1):58-69.

Products are for research use only. Not for human use.

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