ML239, Breast Cancer Stem Cell Inhibitor

ML239 is a potent and selective breast cancer stem cell inhibitor with IC50 ~1.16 μM. It displayed greater than 23-fold selective inhibition of the breast CSC-like cell line (HMLE_sh_Ecad) over the isogenic control cell line (HMLE_sh_GFP). It did not significantly inhibit tumorsphere formation in the breast cancer cell line SUM159, which is a mixture of CSCs and differentiated cells. ML239 is also cytotoxic towards MDA-MB-231 breast cancer cells.

Product information

CAS Number: 1378872-36-6

Molecular Weight: 346.60

Formula: C13H10Cl3N3O2

Chemical Name: (E)-N'-((1H-pyrrol-2-yl)methylene)-2-(2,4,6-trichlorophenoxy)acetohydrazide

Smiles: O=C(COC1C(Cl)=CC(Cl)=CC=1Cl)N/N=C/C1=CC=CN1

InChiKey: NVEDPFICKAIHKD-NGYBGAFCSA-N

InChi: InChI=1S/C13H10Cl3N3O2/c14-8-4-10(15)13(11(16)5-8)21-7-12(20)19-18-6-9-2-1-3-17-9/h1-6,17H,7H2,(H,19,20)/b18-6+

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

ML239 was used at 10 µM final concentration in vitro and in cellular assays.

References:

1. Carmody LC, et al. Phenotypic high-throughput screening elucidates target pathway in breast cancer stem cell-like cells. (2012) J Biomol Screen. 17(9):1204-10.
2. Germain AR, et al. Identification of a selective small molecule inhibitor of breast cancer stem cells. (2012) Bioorg Med Chem Lett. 22(10):3571-4.

Products are for research use only. Not for human use.

Documents