OAC1 --- Oct4 Activator

OAC1 is a potent and selective small molecule to activate both Oct4 and Nanog promoter-driven luciferase reporter genes, identified from a cell-based high-throughput screening. When added to the reprogramming media along with the four reprogramming factors (Oct4, Sox2, Klf4, and c-Myc), OAC1 can significantly enhance iPSC reprogramming efficiency (20-fold higher than that induced by the 4F alone) and accelerate the reprogramming process. The iPSC colonies derived using OAC1 along with the four factors exhibited typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency in a unique manner, independent of either inhibition of the p53-p21 pathway or activation of the Wnt-β-catenin signaling. OAC1 increases transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. OAC1 may be used for large-scale iPSC generation for various applications.

Product information

CAS Number: 300586-90-7

Molecular Weight: 237.26

Formula: C14H11N3O

Chemical Name: N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide

Smiles: O=C(NC1C=C2C=CNC2=CN=1)C1C=CC=CC=1

InChiKey: HWJRIFZDXJKJJN-UHFFFAOYSA-N

InChi: InChI=1S/C14H11N3O/c18-14(10-4-2-1-3-5-10)17-13-8-11-6-7-15-12(11)9-16-13/h1-9,15H,(H,16,17,18)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

OAC1 was used at 1 µM final concentration in iPSC culture media for 7 days (2 days after viral transduction with the four reprogramming factors).

References:

1. Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. (2012) Proc Natl Acad Sci USA.109(51):20853-8.

Products are for research use only. Not for human use.

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