PS48 --- PDK1 Activator

PS48 is a novel PDK1 (phosphoinositide-dependent protein kinase 1) activator (Kd = 10.3 μM), which binds to the HM/PIF binding pocket rather than the ATP-binding site. PS48 has been used to enhance iPSC reprogramming from human epidermal keratinocytes and endothelial cells.

Product information

CAS Number: 1180676-32-7

Molecular Weight: 286.75

Formula: C17H15ClO2

Synonym:

PS-48

Chemical Name: 5-(4-Chloro-phenyl)-3-phenyl-pent-2-enoic acid

Smiles: OC(=O)C=C(CCC1C=CC(Cl)=CC=1)C1C=CC=CC=1

InChiKey: LLJYFDRQFPQGNY-QINSGFPZSA-N

InChi: InChI=1S/C17H15ClO2/c18-16-10-7-13(8-11-16)6-9-15(12-17(19)20)14-4-2-1-3-5-14/h1-5,7-8,10-12H,6,9H2,(H,19,20)/b15-12-

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

PS48 was used at 5 µM concentration in cell culture.

References:

1. Hindie V, et al. Structure and allosteric effects of low-molecular- weight activators on the protein kinase PDK1. (2009) Nature Chem Biol. 5: 758-764.
2. Stroba A, et al. 3,5-Diphenylpent-2-enoic acids as allosteric activators of the protein kinase PDK1: structure-activity relationships and thermodynamic characterization of binding as paradigms for PIF-binding pocket-targeting compounds. (2009) J Med Chem. 52(15):4683-93.
3. Zhu S, et al. Reprogramming of Human Primary Somatic Cells by Oct4 and Chemical Compounds. (2010) Cell Stem Cell. 7 (6)651-655.

Products are for research use only. Not for human use.

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