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TAME, Ubiquitin Ligase APC/C Inhibitor

TAME, Ubiquitin Ligase APC/C Inhibitor

$29.00
A competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C).
Catalog No. Unit Price Qty
M60246-2s 2 mg solid $29.00

Details

Product Information
Molecular Weight: 378.87
Formula: C14H22N4O4S.HCl
Purity: ≥98%
CAS#: 1784-03-8
Solubility: DMSO and water up to 100 mM
Chemical Name: (S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.

How to Use:


In vitro:
TAME was used at 50-200 µM final concentration in various in vitro assays.
In vivo: n/a

Reference:

  • 1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95. 
  • 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92. 


TAME_spec.pdf    
TAME_MSDS.pdf  

Products are for research use only. Not for human use. 

Description

Details

Product Information
Molecular Weight: 378.87
Formula: C14H22N4O4S.HCl
Purity: ≥98%
CAS#: 1784-03-8
Solubility: DMSO and water up to 100 mM
Chemical Name: (S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.

How to Use:


In vitro:
TAME was used at 50-200 µM final concentration in various in vitro assays.
In vivo: n/a

Reference:

  • 1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95. 
  • 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92. 


TAME_spec.pdf    
TAME_MSDS.pdf  

Products are for research use only. Not for human use. 

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