WDR5-C47 --- WDR5-MLL (SET1) Antagonist

WDR5-C47 is a highly potent and selective antagonist of WDR5-MLL interaction with an IC50 ~0.3 µM. WDR5-C47 was designed based on the structure of the WDR5-MLL complex. The WD40-repeat protein WDR5 plays a critical role in maintaining the integrity of the MLL complex and fully activating its methyltransferase function. The MLL complex, the trithorax-like family of SET1 methyltransferase, catalyzes trimethylation of lysine 4 on histone 3, and they have been widely implicated in various cancers. WDR5-C47 could serve as a tool compound to study functions of SET1-family of human histone methyltransferases and further develop therapeutic drug molecules targeting the WDR5−MLL interaction.

Product information

CAS Number: 1422389-91-0

Molecular Weight: 406.84

Formula: C19H20ClFN4O3

Chemical Name: 2-chloro-4-fluoro-3-methyl-N-(2-(4-methylpiperazin-1-yl)-5-nitrophenyl)benzamide

Smiles: CN1CCN(CC1)C1=CC=C(C=C1NC(=O)C1=CC=C(F)C(C)=C1Cl)[N+]([O-])=O

InChiKey: QHWMIGPLLWYRIR-UHFFFAOYSA-N

InChi: InChI=1S/C19H20ClFN4O3/c1-12-15(21)5-4-14(18(12)20)19(26)22-16-11-13(25(27)28)3-6-17(16)24-9-7-23(2)8-10-24/h3-6,11H,7-10H2,1-2H3,(H,22,26)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

WDR5-C47 was suggested to be used at 10 µM final concentration in vitro and in cellular assays.

References:

1. Bolshan Y, et al. Synthesis, Optimization, and Evaluation of Novel Small Molecules as Antagonists of WDR5-MLL Interaction. (2013) ACS Med. Chem. Lett., 4 (3), 353–357.

Products are for research use only. Not for human use.

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