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Azidothymidine (AZT), Reverse Transcriptase Inhibitor

Azidothymidine (AZT), Reverse Transcriptase Inhibitor

$29.00
A selective, orally bioavailable and brain penetrant reverse transcriptase inhibitor.
Catalog No. Unit Price Qty
M60238-2s 2 mg solid $29.00

Details

Product Information
Molecular Weight: 267.24
Formula: C10H13N5O4
Purity: ≥98%
CAS#: 30516-87-1
Solubility: DMSO up to 100 mM, Water up to 50 mM
Chemical Name: 1-((2R,4S,5S)-4-azido-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

Azidothymidine (AZT) is a selective, orally bioavailable and brain penetrant reverse transcriptase inhibitor. It has 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. It can suppress HIV-1 replication and enhance cell viability in a HIV-1 infected T cell line. It can also suppress growth of a multiple myeloma (MM) cell line and reduces the growth of MM tumor xenografts in mice. In recent studies, AZT can enhance CRISPR-mediated sequence-specific gene knockout via NHEJ in human induced pluripotent stem cells (iPSCs) and other cell types.

How to Use:

In vitro: Azidothymidine (AZT) was used at 5-30 µM final concentration in various in vitro assays.
In vivo: Azidothymidine (AZT) was dosed to mice at 10-50 mg/kg orally once per day.

Reference:

  • 1. Mitsuya H, et al. 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. (1985) Proc Natl Acad Sci USA. 82(20):7096-100.
  • 2. Furman PA, et al. Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. (1986) Proc Natl Acad Sci USA. 83(21):8333-7.
  • 3. Broder S, et al. The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. (2010) Antiviral Res. 85(1):1-18. 
  • 4. Pereira J, et al. Azidothymidine is effective against human multiple myeloma: a new use for an old drug? (2013) Anticancer Agents Med Chem. 13(1):186-92.
  • 5. Yu C, et al. Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. (2015) Cell Stem Cell 16(2):142-7. 

Azidothymidine_spec.pdf    


Products are for research use only. Not for human use. 

Description

Details

Product Information
Molecular Weight: 267.24
Formula: C10H13N5O4
Purity: ≥98%
CAS#: 30516-87-1
Solubility: DMSO up to 100 mM, Water up to 50 mM
Chemical Name: 1-((2R,4S,5S)-4-azido-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

Azidothymidine (AZT) is a selective, orally bioavailable and brain penetrant reverse transcriptase inhibitor. It has 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. It can suppress HIV-1 replication and enhance cell viability in a HIV-1 infected T cell line. It can also suppress growth of a multiple myeloma (MM) cell line and reduces the growth of MM tumor xenografts in mice. In recent studies, AZT can enhance CRISPR-mediated sequence-specific gene knockout via NHEJ in human induced pluripotent stem cells (iPSCs) and other cell types.

How to Use:

In vitro: Azidothymidine (AZT) was used at 5-30 µM final concentration in various in vitro assays.
In vivo: Azidothymidine (AZT) was dosed to mice at 10-50 mg/kg orally once per day.

Reference:

  • 1. Mitsuya H, et al. 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. (1985) Proc Natl Acad Sci USA. 82(20):7096-100.
  • 2. Furman PA, et al. Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. (1986) Proc Natl Acad Sci USA. 83(21):8333-7.
  • 3. Broder S, et al. The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. (2010) Antiviral Res. 85(1):1-18. 
  • 4. Pereira J, et al. Azidothymidine is effective against human multiple myeloma: a new use for an old drug? (2013) Anticancer Agents Med Chem. 13(1):186-92.
  • 5. Yu C, et al. Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. (2015) Cell Stem Cell 16(2):142-7. 

Azidothymidine_spec.pdf    


Products are for research use only. Not for human use. 

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