Azidothymidine (AZT) --- Reverse Transcriptase Inhibitor

Azidothymidine (AZT) is a selective, orally bioavailable and brain penetrant reverse transcriptase inhibitor. It has 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. It can suppress HIV-1 replication and enhance cell viability in a HIV-1 infected T cell line. It can also suppress growth of a multiple myeloma (MM) cell line and reduces the growth of MM tumor xenografts in mice. In recent studies, AZT can enhance CRISPR-mediated sequence-specific gene knockout via NHEJ in human induced pluripotent stem cells (iPSCs) and other cell types.

Product information

CAS Number: 30516-87-1

Molecular Weight: 267.24

Formula: C10H13N5O4

Chemical Name: 1-((2R,4S,5S)-4-azido-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione

Smiles: CC1=CN([C@H]2C[C@H](N=[N+]=[N-])[C@@H](CO)O2)C(=O)NC1=O

InChiKey: HBOMLICNUCNMMY-XLPZGREQSA-N

InChi: InChI=1S/C10H13N5O4/c1-5-3-15(10(18)12-9(5)17)8-2-6(13-14-11)7(4-16)19-8/h3,6-8,16H,2,4H2,1H3,(H,12,17,18)/t6-,7+,8+/m0/s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM, Water up to 50 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Azidothymidine (AZT) was used at 5-30 µM final concentration in various in vitro assays.

In Vivo:

Azidothymidine (AZT) was dosed to mice at 10-50 mg/kg orally once per day.

References:

1. Mitsuya H, et al. 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. (1985) Proc Natl Acad Sci USA. 82(20):7096-100.
2. Furman PA, et al. Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. (1986) Proc Natl Acad Sci USA. 83(21):8333-7.
3. Broder S, et al. The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. (2010) Antiviral Res. 85(1):1-18.

Products are for research use only. Not for human use.

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