α-Conotoxin Vc1.1 TFA

α-Conotoxin Vc1.1 TFA is a disulfide-bonded peptide isolated from Conus victoriae and is a selective nAChR antagonist. α-Conotoxin Vc1.1 TFA inhibits α3α5β2, α3β2 and α3β4 with IC50s of 7.2 μM, 7.3 μM and 4.2 μM, respectively, and has less inhibitory effect on other nAChR subtypes. α-Conotoxin Vc1.1 TFA has the potential for neuropathic pain reserach.

Product information

Molecular Weight: 1921.00

Formula: C73H104F3N23O27S4

Chemical Name: 2, 2'-((6S, 9S, 12R, 17R, 20S, 23S, 25aS, 31S, 34S, 37S, 40S, 43R, 46S, 48aS, 53R)-31-((1H-imidazol-4-yl)methyl)-40-(2-amino-2-oxoethyl)-53-(2-aminoacetamido)-20-((S)-sec-butyl)-17-carbamoyl-23-(2-carboxyethyl)-46-(3-guanidinopropyl)-37-(4-hydroxybenzyl)-9-(hydroxymethyl)-5, 8, 11, 19, 22, 25, 30, 33, 36, 39, 42, 45, 48, 54-tetradecaoxotetratetracontahydro-1H, 5H-12, 43-(epiminopropanodithiomethano)dipyrrolo[2, 1-h1:2', 1'-m][1, 2]dithia[5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41]tridecaazacyclotetratetracontine-6, 34-diyl)diacetic acid--2, 2, 2-trifluoroacetic acid (1/1)

Smiles: C[C@@H](CC)[C@@H]1NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H]2CCCN2C(=O)[C@H](CC2=CNC=N2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC2C=CC(O)=CC=2)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]2CSSC[C@H](NC(=O)CN)C(=O)N[C@@H](CSSC[C@H](NC1=O)C(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)N2.OC(=O)C(F)(F)F

InChiKey: WEZFNKWMAQIZFP-PFVJWRALSA-N

InChi: InChI=1S/C71H103N23O25S4.C2HF3O2/c1-3-32(2)55-68(117)89-44(56(74)105)27-120-122-30-47-65(114)88-43(26-95)62(111)87-42(23-54(103)104)70(119)94-18-6-8-48(94)66(115)81-36(7-4-16-78-71(75)76)57(106)90-46(29-123-121-28-45(63(112)91-47)80-51(98)24-72)64(113)84-39(21-50(73)97)60(109)83-38(19-33-10-12-35(96)13-11-33)59(108)85-40(22-53(101)102)61(110)86-41(20-34-25-77-31-79-34)69(118)93-17-5-9-49(93)67(116)82-37(58(107)92-55)14-15-52(99)100;3-2(4,5)1(6)7/h10-13,25,31-32,36-49,55,95-96H,3-9,14-24,26-30,72H2,1-2H3,(H2,73,97)(H2,74,105)(H,77,79)(H,80,98)(H,81,115)(H,82,116)(H,83,109)(H,84,113)(H,85,108)(H,86,110)(H,87,111)(H,88,114)(H,89,117)(H,90,106)(H,91,112)(H,92,107)(H,99,100)(H,101,102)(H,103,104)(H4,75,76,78);(H,6,7)/t32-,36-,37-,38-,39-,40-,41-,42-,43-,44-,45-,46-,47-,48-,49-,55-;/m0./s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

The α-Conotoxin Vc1.1 is first discovered using a PCR screen of cDNAs from the venom ducts of Conus victoriae. α-Conotoxin Vc1.1 inhibits nicotine-evoked membrane currents in isolated bovine chromaffin cells in a concentration-dependent manner and preferentially targets peripheral nAChR subtypes over central subtypes. The three-dimensional structure of Vc1.1 comprises a small alpha-helix spanning residues Pro6 to Asp11 and is braced by the I-III, II-IV disulfide connectivity seen in other alpha-conotoxins. The cysteine spacing within the sequence of α-Conotoxin Vc1.1 suggests that it is a member of the 4/7 subclass of α-conotoxins, which includes the extensively studied conotoxins MII, EpI and PnIB.

In Vivo:

α-Conotoxin Vc1.1 (0.18-18 μg/μL; intramuscular injection; daily; for 7 days; male Sprague-Dawley rats) treatment suppresses pain behaviors and also accelerates functional recovery of injured neurons in CCI rats.

Products are for research use only. Not for human use.