Description
WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells.
Product information
CAS Number: 150849-52-8
Molecular Weight: 651.34
Formula: C19H11IN5NaO8S2
Chemical Name: sodium 5-(2,4-disulfonatophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-3H-1,2λ⁵,3,4-tetrazol-2-ylium
Smiles: [Na+].[O-][N+](=O)C1C=CC(=CC=1)N1N=C(N=[N+]1C1C=CC(I)=CC=1)C1=CC=C(C=C1S([O-])(=O)=O)S([O-])(=O)=O
InChiKey: JUJBNYBVVQSIOU-UHFFFAOYSA-M
InChi: InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C). H2O : < 0.1 mg/mL (ultrasonic;warming;adjust pH to 9 with NaOH;heat to 80°C) (insoluble).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
WST-1 is used for a wide range of assays that include cell viability, proliferation, plasma membrane electron transport system, superoxide generation, superoxide dismutase, and xanthine oxidase assay. A water-soluble formazan dye is produced from the reduction of WST-1 tetrazolium, of which optical density at 450 nm is measured to evaluate cell viability.
Products are for research use only. Not for human use.
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