Description
Hoechst 34580 tetrahydrochloride is a cell-permeable fluorescent dye for staining DNA and nuclei.
Product information
Molecular Weight: 597.41
Formula: C27H33Cl4N7
Chemical Name: N,N-dimethyl-4-{5-[5-(4-methylpiperazin-1-yl)-1H-1,3-benzodiazol-2-yl]-1H-1,3-benzodiazol-2-yl}aniline; tetrakis(chlorohydrogen)
Smiles: Cl.Cl.Cl.Cl.CN(C)C1C=CC(=CC=1)C1NC2=CC=C(C=C2N=1)C1NC2=CC=C(C=C2N=1)N1CCN(C)CC1
InChiKey: CLWSVNOLFQKMQY-UHFFFAOYSA-N
InChi: InChI=1S/C27H29N7.4ClH/c1-32(2)20-7-4-18(5-8-20)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-9-21(17-25(23)31-27)34-14-12-33(3)13-15-34;;;;/h4-11,16-17H,12-15H2,1-3H3,(H,28,30)(H,29,31);4*1H
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: H2O : 2.67 mg/mL (4.47 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC50 of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC50) is examined. The IC50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580.
Products are for research use only. Not for human use.
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