Hemin - CAS# 16009-13-5

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

Product information

CAS Number: 16009-13-5

Molecular Weight: 651.94

Formula: C34H32ClFeN4O4

Synonym:

Hemin chloride

Chemical Name: 3-[18-(2-carboxyethyl)-8, 13-bis(ethenyl)-3, 7, 12, 17-tetramethylporphyrin-21, 24-diid-2-yl]propanoic acid;iron(3+);chloride

Smiles: [Cl-].[Fe+3].CC1C2=CC3=NC(=CC4=NC(=CC5[N-]C(C=C([N-]2)C=1CCC(O)=O)=C(CCC(O)=O)C=5C)C(C=C)=C4C)C(C=C)=C3C

InChiKey: BTIJJDXEELBZFS-HXFTUNQESA-K

InChi: InChI=1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 17.33 mg/mL (26.58 mM; Need ultrasonic) NH4OH : 2.5 mg/mL (3.83 mM; ultrasonic and adjust pH to 11 with H2O) H2O : 0.1 mg/mL (0.15 mM; Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %, 51±3 %, and 38±8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment.

In Vivo:

Following the i.p. administration of Hemin (100 μmol/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning.

References:

1. Vanova K, et al. Heme oxygenase is not involved in the anti-proliferative effects of statins on pancreatic cancer cells. BMC Cancer. 2016 May 12;16:309.
2. Chen HH, et al. Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2195-201.

Products are for research use only. Not for human use.