Tautomycetin - CAS# 119757-73-2

Tautomycetin is a potent and specifical PP1 inhibitor with the potential apoptosis-inducing activity. Tautomycetin inhibits purified PP1 and PP2A enzymes with IC50s of 1.6 nM and 62 nM, respectively. Tautomycetin is an antifungal antibiotic and has immunosuppressive effects in vivo. Tautomycetin can be used as a novel powerful tool to elucidate the physiological roles of PP1 in various biological events.

Product information

CAS Number: 119757-73-2

Molecular Weight: 606.74

Formula: C33H50O10

Chemical Name: (16E)-17-ethyl-4,8-dihydroxy-3,7,11,13-tetramethyl-6,15-dioxononadeca-16,18-dien-2-yl 3-hydroxy-3-(4-methyl-2,5-dioxo-2,5-dihydrofuran-3-yl)propanoate

Smiles: CC1C(=O)OC(=O)C=1C(O)CC(=O)OC(C)C(C)C(O)CC(=O)C(C)C(O)CCC(C)CC(C)CC(=O)/C=C(\CC)/C=C

InChiKey: VAIBGAONSFVVKI-IWIPYMOSSA-N

InChi: InChI=1S/C33H50O10/c1-9-24(10-2)15-25(34)14-19(4)13-18(3)11-12-26(35)21(6)28(37)16-27(36)20(5)23(8)42-30(39)17-29(38)31-22(7)32(40)43-33(31)41/h9,15,18-21,23,26-27,29,35-36,38H,1,10-14,16-17H2,2-8H3/b24-15-

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Tautomycetin (100 ng/ml-10 μg/ml; 48 hours) has inhibitory effects on T cell proliferation in the murine mixed lymphocyte reaction (MLR) assays. The level of inhibition of MLR by TMC is 100-fold higher than that by CsA, and the IC50 of TMC is 7.8 nM. Tautomycetin (1 μg/ml) possesses immunosuppressive activity to inhibit the proliferation of T cells by inhibition of IL-2 secretion in human primary T cells. Tautomycetin (1 μg/ml; pretreated for 5 hours) blocks the phosphorylation of tyrosine residues on several specific cellular proteins in a dose- and time-dependent manner in T cells stimulated by immobilized OKT3 mAb. Tautomycetin (1 μg/ml; 5 hours) significantly inhibits the phosphorylation of Akt and BAD in a dose-dependent fashion inn Jurkat TAg cell line.

In Vivo:

Tautomycetin (intraperitoneal injection; 0.03 mg/kg-5 mg/kg; 30 days) exhibits in vivo immunosuppressive effect in in vivo heart allograft transplantation. And the biochemical analysis of GOP, GTP, glucose, and creatine levels in blood after administration of TMC up to 5 mg/kg in rats for 30 days does not show any significant liver and kidney toxicity in rats.

Products are for research use only. Not for human use.