β-Amyloid (1-28)

β-Amyloid (1-28) is a β-Amyloid protein fragment involved in metal binding. Beta-amyloid is a peptide that forms amyloid plaques in the brains of Alzheimer's disease (AD) patients.

Product information

CAS Number: 109770-29-8

Molecular Weight: 3262.46

Formula: C145H209N41O46

Chemical Name: 44, 47, 68-tris((1H-imidazol-4-yl)methyl)-83-amino-5-(2-amino-2-oxoethyl)-41-(3-amino-3-oxopropyl)-2, 38-bis(4-aminobutyl)-26, 29, 74-tribenzyl-20, 53, 77-tris(2-carboxyethyl)-17, 65-bis(carboxymethyl)-71-(3-guanidinopropyl)-56-(4-hydroxybenzyl)-8, 62-bis(hydroxymethyl)-35-isobutyl-14, 32, 50-triisopropyl-23, 80-dimethyl-4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82-heptacosaoxo-3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81-heptacosaazapentaoctacontane-1, 85-dioic acid

Smiles: CC(C)CC(NC(=O)C(CCCCN)NC(=O)C(CCC(N)=O)NC(=O)C(CC1=CNC=N1)NC(=O)C(CC1=CNC=N1)NC(=O)C(NC(=O)C(CCC(O)=O)NC(=O)C(CC1C=CC(O)=CC=1)NC(=O)CNC(=O)C(CO)NC(=O)C(CC(O)=O)NC(=O)C(CC1=CNC=N1)NC(=O)C(CCCNC(N)=N)NC(=O)C(CC1C=CC=CC=1)NC(=O)C(CCC(O)=O)NC(=O)C(C)NC(=O)C(N)CC(O)=O)C(C)C)C(=O)NC(C(C)C)C(=O)NC(CC1C=CC=CC=1)C(=O)NC(CC1C=CC=CC=1)C(=O)NC(C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(O)=O)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CO)C(=O)NC(CC(N)=O)C(=O)NC(CCCCN)C(O)=O

InChiKey: CMKVKXQGLWBAFY-UHFFFAOYSA-N

InChi: InChI=1S/C145H209N41O46/c1-71(2)49-93(138(225)186-118(74(7)8)142(229)181-97(52-79-29-18-13-19-30-79)132(219)175-95(50-77-25-14-11-15-26-77)129(216)163-76(10)120(207)167-90(40-44-111(196)197)127(214)179-103(60-115(204)205)139(226)184-116(72(3)4)141(228)158-65-109(193)165-105(67-188)140(227)178-101(58-107(150)191)136(223)172-92(144(231)232)32-21-23-47-147)173-123(210)86(31-20-22-46-146)168-125(212)88(38-42-106(149)190)170-133(220)98(54-81-61-153-68-159-81)177-135(222)100(56-83-63-155-70-161-83)182-143(230)117(73(5)6)185-128(215)91(41-45-112(198)199)171-130(217)94(53-80-34-36-84(189)37-35-80)164-108(192)64-157-122(209)104(66-187)183-137(224)102(59-114(202)203)180-134(221)99(55-82-62-154-69-160-82)176-124(211)87(33-24-48-156-145(151)152)169-131(218)96(51-78-27-16-12-17-28-78)174-126(213)89(39-43-110(194)195)166-119(206)75(9)162-121(208)85(148)57-113(200)201/h11-19,25-30,34-37,61-63,68-76,85-105,116-118,187-189H,20-24,31-33,38-60,64-67,146-148H2,1-10H3,(H2,149,190)(H2,150,191)(H,153,159)(H,154,160)(H,155,161)(H,157,209)(H,158,228)(H,162,208)(H,163,216)(H,164,192)(H,165,193)(H,166,206)(H,167,207)(H,168,212)(H,169,218)(H,170,220)(H,171,217)(H,172,223)(H,173,210)(H,174,213)(H,175,219)(H,176,211)(H,177,222)(H,178,227)(H,179,214)(H,180,221)(H,181,229)(H,182,230)(H,183,224)(H,184,226)(H,185,215)(H,186,225)(H,194,195)(H,196,197)(H,198,199)(H,200,201)(H,202,203)(H,204,205)(H,231,232)(H4,151,152,156)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.

In Vivo:

β-Amyloid (1-28) fragment is considered as valid models to examine the contribution of the key histidine residues (His , His in mouse and His , His , His in human fragments) to the Ab–Cu2+ interaction. Copper binds to β-Amyloid (1-28) at pH 7.4 with an affinity of Ka10 7 M-1.

Products are for research use only. Not for human use.