Description
W146 is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.
Product information
CAS Number: 909725-61-7
Molecular Weight: 342.37
Formula: C16H27N2O4P
Chemical Name: [(3R)-3-amino-3-[(3-hexylphenyl)carbamoyl]propyl]phosphonic acid
Smiles: CCCCCCC1C=C(C=CC=1)NC(=O)[C@H](N)CCP(O)(O)=O
InChiKey: FWJRVGZWNDOOFH-OAHLLOKOSA-N
InChi: InChI=1S/C16H27N2O4P/c1-2-3-4-5-7-13-8-6-9-14(12-13)18-16(19)15(17)10-11-23(20,21)22/h6,8-9,12,15H,2-5,7,10-11,17H2,1H3,(H,18,19)(H2,20,21,22)/t15-/m1/s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
W146 is a S1PR1 antagonist with a Ki of ~70-80 nM. Compared with the S1P treated group, the S1P attenuated TUNEL labeling index of EPCs is significantly increased by pretreatment with W146. Furthermore, pretreatment with W146 significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146.
In Vivo:
Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice. It is found that a significant increase in KSL-HSPC mobilization when mice are pretreated with W146, compared to that in mice pretreated with dextran followed by AMD3100 administration. Moreover, pre-treatment of W146 shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone. The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization.
Products are for research use only. Not for human use.
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