Methoxy-X04 --- Fluorescent Amyloid β (Aβ) Probe

Methoxy-X04 is a fluorescent amyloid β (Aβ) probe for the detection and quantification of plaques, tangles and cerebrovascular amyloid with good specificity. It is a derivative of Congo red and Chrysamine-G that contains no acid groups and is therefore smaller and much more lipophilic than Congo red or Chrysamine-G. Methoxy-X04 retains in vitro binding affinity for amyloid beta (Abeta) fibrils (Ki = 26.8 nM) very similar to that of Chrysamine-G. Using multiphoton microscopy to obtain high-resolution (1 microm) fluorescent images from the brains of living PSI/APP mice, individual plaques could be distinguished within 30 to 60 min after a single IV injection or a single IP injection of Methoxy-X04.

Product information

CAS Number: 863918-78-9

Molecular Weight: 344.40

Formula: C23H20O3

Chemical Name: 4,4'-((1E,1'E)-(2-methoxy-1,4-phenylene)bis(ethene-2,1-diyl))diphenol

Smiles: COC1=CC(/C=C/C2C=CC(O)=CC=2)=CC=C1/C=C/C1C=CC(O)=CC=1

InChiKey: FGYNZFHVGOFCMD-KHVHPYDTSA-N

InChi: InChI=1S/C23H20O3/c1-26-23-16-19(3-2-17-6-12-21(24)13-7-17)5-11-20(23)10-4-18-8-14-22(25)15-9-18/h2-16,24-25H,1H3/b3-2+,10-4+

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Methoxy-X04 was used at 50 µM final concentration in various in vitro assays.

In Vivo:

Methoxy-X04 was administered to mice by a single IV injection of 5 to 10 mg/kg or by a single IP injection of 10 mg/kg to produce high contrast images of plaques and cerebrovascular amyloid in PSI/APP mouse brain.

References:

1. Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. (2002) J Neuropathol Exp Neurol. 61(9):797-805.
2. Sadowski M, Targeting prion amyloid deposits in vivo. (2004) J Neuropathol Exp Neurol. 63(7):775-84.
3. Bolmont T, et al. Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. (2008) J Neurosci. 28(16):4283-92.
4. Dong J, et al. Multiphoton in vivo imaging of amyloid in animal models of Alzheimer's disease. (2010) Neuropharmacology. 59(4-5):268-75.

Products are for research use only. Not for human use.

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