Guanidine Hydrochloride

2-Hexanone ketone is an industrial solvent which causes nervous system degeneration. MBK is an acronym often used to refer to it. It is used as a general solvent and in paints. It dissolves cellulose nitrate, vinyl polymers and copolymers, and natural and synthetic resins.

Product information

CAS Number: 50-01-1

Molecular Weight: 95.53

Formula: CH6ClN3

Synonym:

Guanidinium chloride

Aminoformamidine Hydrochloride

Chemical Name: guanidine hydrochloride

Smiles: Cl.NC(N)=N

InChiKey: PJJJBBJSCAKJQF-UHFFFAOYSA-N

InChi: InChI=1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 100 mg/mL (1046.79 mM); H2O : 50 mg/mL (523.40 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Guanidine hydrochloride at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state. A considerable degree of ordered structure remains due to the presence of disulfide bonds, which Guanidine hydrochloride does not affect. Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase.

In Vivo:

A significant reduction in the death rate of infant mice infected with ten LD50) of coxsackievirus A16 is observed when they are treated 58 h after infection with two injections of Guanidine hydrochloride at 145 mg/kg per injection.

References:

1. Y Hagihara, et al. Guanidine hydrochloride-induced folding of proteins. J Mol Biol. 1993 May 20;231(2):180-4.
2. Saeed Emadi, et al. A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation. Biochem Biophys Res Commun. 2014 Aug 8;450(4):1339-44.
3. G Jung, et al. Guanidine hydrochloride inhibits Hsp104 activity in vivo: a possible explanation for its effect in curing yeast prions. Curr Microbiol. 2001 Jul;43(1):7-10.

Products are for research use only. Not for human use.